Multiplex PCR for Identification of Bacterial Pathogens of Infectious Pneumonia

Identification of Pathogens in Nasopharyngeal Secretions of Patients with and without Pneumonia by Multiplex RT-PCR Method

        Background and purpose: Pneumonia is the most common infectious cause of death. This study aimed at investigating the causative agent of pneumonia in nasopharyngeal secretions by PCR method. Materials and methods: This case-control study was carried out in patients (older than 19 years of age) suspected of pneumonia admitted to Qaemshahr Razi Hospital and Sari Imam Khomeini Hospital...

The role of multiplex PCR test in identification of bacterial pathogens in lower respiratory tract infections

OBJECTIVES Lower respiratory tract infection is one of the most important causes of morbidity and mortality. However establishing a microbial diagnosis for patients with lower respiratory tract infection is still challenging and is often achieved in only half of cases by conventional methods. This study was designed to compare the fast responsive PCR method with the culture method in lower resp...

Serotype Identification of Recent Iranian Isolates of Infectious Bronchitis Virus by Type-Specific Multiplex RT-PCR

A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Viral and Bacterial Pathogens of Infectious Diarrhea

Diarrhea caused by viral and bacterial infections is a major health problem in developing countries. The purpose of this study is to develop a two-tube multiplex PCR assay using automatic electrophoresis for simultaneous detection of 13 diarrhea-causative viruses or bacteria, with an intended application in provincial Centers for Diseases Control and Prevention, China. The assay was designed to...

Simultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass Spectrometry

Citation: Zhang C, Xiu L, Xiao Y, Xie Z, Ren L and Peng J (2018) Simultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass Spectrometry. Front. Cell. Infect. Microbiol. 8:107. doi: 10.3389/fcimb.2018.00107 Simultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass S...